Computational and Mathematical Methods in Medicine
Volume 2013 (2013), Article ID 890203, 8 pages
http://dx.doi.org/10.1155/2013/890203
Research Article

A New Multichannel Spectral Imaging Laser Scanning Confocal Microscope

Yunhai Zhang, Bian Hu, Yakang Dai, Haomin Yang, Wei Huang, Xiaojun Xue, Fazhi Li, Xin Zhang, Chenyu Jiang, Fei Gao, and Jian Chang

Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China

Received 3 January 2013; Accepted 24 February 2013

Academic Editor: Jimin Liang

Copyright © 2013 Yunhai Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We have developed a new multichannel spectral imaging laser scanning confocal microscope for effective detection of multiple fluorescent labeling in the research of biological tissues. In this paper, the design and key technologies of the system are introduced. Representative results on confocal imaging, 3-dimensional sectioning imaging, and spectral imaging are demonstrated. The results indicated that the system is applicable to multiple fluorescent labeling in biological experiments.